Цитата:
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Сообщение от V. ZAITSEV
Что касается существа вопроса, то напомню часть из уже приводимых (и ни раз!) по этой патологии материалов. Может хотя бы на этот раз у кого-нибудь из оппонентов, объясняющих мне, какая лажа эта озонотерапия, найдется немного времени, чтобы их прочесть.
OZONE PROPHYLACTIC EFFECT AND ANTIBIOTICS AS A MODULATOR OF INFLAMATORY SEPTIC PROCESS IN RATS.
2Institute of Anatomy Celular and Molecular Biology, University od Marburg, Germany.
3Intitute of Microbiology, University od Marburg, Germany.
4Institute of Animals Laboratory, University od Marburg, Germany.
Zamora Z.B.1, Menéndez S.1, Bette M.2, Mutters R.3, Hoffmann S.4 and Schulz S.4.
1Ozone Research Center, Cuba.
ABSTRACT
192 Wistar male rats (180 - 200 g) were used to evaluate the prophylactic effect of ozone combined with antibiotics in septic shock model. The proinflammatory cytokines (IL-1b, TNFa, IL-6 and IL-2) expression were also determinated in different haemathopoyetic organs. The animals were taken at random and divided into 16 groups, 12 animals each: Group A, positive control (sepsis without treatment); groups B and C treated with ozone (200 mg / 250 g b. w) and Cefotaxim (25 mg/kg), (0h, +1h) and (+1 h) respectivitly; groups D and E, ozone and Cefodizim (25 mg/kg (0h, +1h) and (+1 h) respectively; groups F and G ozone and levofloxacin (12.5 mg/kg), (0h, +1h) and (+1 h) respectively; groups H and I ozone and Piperacillin /Tazobactam (65mg/kg), (0h, +1h) and (+1 h) respectively. The 7septic shock was carried out by single intraperitoneal injection of fecal material (0.65 g/kg b/w) from a donor rat, diluted in saline solution 0.9 %. The other groups (J-P) of animals were treated only with antibiotics.
The group pretreated with ozone and antibiotics showed a significant increase in the survival rate and showed also a decrease of IL-1 mRNA expression in liver. Ozone pretreatment might prevent lethal peritonitis by controlling inflammatory process generated physiologically into the body against specific damage or specific organism.
В принципе, можно привести ещё ни один десяток аналогичных работ, но не хочу слишком сильно портить воскресное настроение у оппонентов.
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Уважаемый Владимир Яковлевич,
Conclusion 1:
Ozone pretreatment might prevent lethal peritonitis by controlling inflammatory process generated physiologically into the body against specific damage or specific organism.
Conclusions 2:
Ozone pre-treatment was pro-inflammatory in sepsis with a trend to reduced survival. Therefore, its effects in sepsis should be further evaluated in animal trials.
Как быть оппонентам? Кому верить? Может проверим, ну хотя бы еще разочек, ну пожалуйста, перед детским то отделением...
Inflamm Res. 2004 Aug;53 Suppl 2:S122-5. Epub 2004 Aug 10. Related Articles, Links
Pre-treatment with ozonized oxygen (O(3)) aggravates inflammation in septic rats.
Torossian A, Ruehlmann S, Eberhart L, Middeke M, Wulf H, Bauhofer A.
Department of Anaesthesia and Critical Care, University of Marburg, Baldingerstrasse 1, 35033, Marburg, Germany, [
Ссылки доступны только зарегистрированным пользователям ].
Objective and design:Ozone is produced by neutrophils during bacterial killing. Its application was found to be beneficial in peritonitis patients. Therefore, we measured survival and cytokines after ozone pre-treatment in septic rats. Subjects and treatment:With approval, 40 male Wistar-rats were allocated to 1) ozone pre-treatment for five days before intra-abdominal sepsis, or 2) no pre-treatment. Methods:The primary endpoint was mortality at 120 h. Secondary endpoints were plasma cytokine levels. Results:In the control group mortality was 50% (10/20 rats). After ozone pre-treatment, survival was only 35% (7/20 rats, Log-Rank test: P = 0.10). Ozone increased TNF-alpha and MIP-2 after infection: 127+/-23 pg/ml and 94+/-19 pg/ml (control group: 398 pg/ml and 369 pg/ml; P < 0.002 and P < 0.01). IL-6 levels were similar in both groups. Conclusions:
Ozone pre-treatment was pro-inflammatory in sepsis with a trend to reduced survival. Therefore, its effects in sepsis should be further evaluated in animal trials.