Proc West Pharmacol Soc. 2002;45:65-7.
EFFECT OF OZONE PRENATAL EXPOSURE ON POSTNATAL DEVELOPMENT OF CEREBELLUM. 1R. M. Romero-Velázquez, 1, 2A. Alfaro-, 2R. González & 1A. González-Maciel.
1Instituto Nacional de Pediatría, 2Instituto de la Comunicación Humana, México, D. F.
Although the functional and biochemical ozone (O3) effects have been examined, little morphological study exists that demonstrates alterations in the central nervous system. The present study analyzes the morphology of the cerebellum of rats of 90 days of age descendants of females exposed to O3 (1.0 ppm for 12h/day, 20:00-08:00 h) during the first sixteen days (G1) or the entire gestation (G2). To obtain control animals (GC), pregnant rats were kept in pollution-free, humidity, temperature and light/dark controlled environmental chambers with ad libitum food and water. The experimental rats were kept in the same conditions, except for O3 exposure, delivered to the chambers by a P15 Triozon generator, and monitored by a 1008-PC Dasibi ultraviolet light analyzer. Rats were deeply anesthetized and perfused transcardially with sodium phosphate buffer, and 10% formalin. The cerebellum in the midsagittal plane was embedding in paraffin in order to proceed with the histological technique. In GC rats no changes were seen in the histological patterns of different cell layers of cerebellum. G1 revealed significantly reduction of Purkinje cells number and the total cerebella cortex area, zones with degenerating Purkinje cells and zones without Purkinje cell. In G1 and G2 damage of the molecular layer and incomplete folding pattern of some lobes was found. The reduced area of the cerebella cortex together with histological changes, might correspond probably to changes in developmental events, by high production of free radicals owing to O3 exposure during gestation.
Bull Exp Biol Med. 2000 Jul;130(7):715-8.
Morphofunctional changes in the placenta after ozone therapy.
Andikyan VM, Voloshchuk IN, Kovganko PA, Clemente JM.
Department of Pathological Anatomy, Therapeutic Faculty, IM Setchenov Moscow Medical Academy.
Macro- and microscopic aspects in the morphology of placenta after ozone therapy were studied in pregnant women with placental insufficiency and the results were compared with the control group. In the main group, the placentas were smaller and weighed less. After ozone therapy enlargement of terminal villi with true syncytiocapillary membranes was noted. A stimulating effect of ozone therapy on the growth and differentiation of terminal villi was established.
Два показательных подхода к проведению исследований: в первом случае исследователи не забыли упомянуть даже в абстракте, что крысы были под глубоким наркозом, а во втором...
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